Expression of themetAgene ofEscherichia coliK-12 in recombinant plasmids
نویسندگان
چکیده
منابع مشابه
CONSTRUCTION OF RECOMBINANT PLASMIDS FOR PERIPLASMIC EXPRESSION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI UNDER T7 AND LAC PROMOTERS
In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the related cDNA was inserted in two expression plasmids carrying pelB signal peptide, one with lac bacterial promoter and the other with a bacteriophage T7-based promoter. The recombinant plasmids were moved to TG1 and BL21 strains of E. coli, respectively. To induce the expression systems, IPTG and...
متن کاملconstruction of recombinant plasmids for periplasmic expression of human growth hormone in escherichia coli under t7 and lac promoters
in order to study the periplasmic expression of human growth hormone (hgh) in escherichia coli, the related cdna was inserted in two expression plasmids carrying pelb signal peptide, one with lac bacterial promoter and the other with a bacteriophage t7-based promoter. the recombinant plasmids were moved to tg1 and bl21 strains of e. coli, respectively. to induce the expression systems, iptg and...
متن کاملExpression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate.
We have studied the effects of sodium butyrate on DNA-mediated gene transfer in an effort to investigate interrelationships between chromatin structure and expression of recombinant plasmids. Our results demonstrate that butyrate affects the early stages of gene activity following DNA uptake at least two levels. First, the number of cells able to express foreign DNA increases from 10% to up to ...
متن کاملPhenotypic expression of recombinant plasmids pKT107 and pHK11 in an avirulent avian Escherichia coli.
An avirulent wild-type avian Escherichia coli strain (Av) was electrotransformed with plasmids coding for complement resistance (pKT107) and Colicin V (ColV) production (pHK11) in order to study the effects of complement resistance and ColV production on virulence. Transformants were also compared with the wild type for embryo lethality, uptake by macrophages, motility, growth rate, plasmid con...
متن کاملDNA supercoiling of recombinant plasmids in mammalian cells.
We have used chloroquine/agarose gel electrophoresis and a blot-hybridization technique to study the modulation of superhelicity of extrachromosomal DNA in mammalian cells. The high sensitivity of the procedure has allowed us to measure the change in the specific linking difference or superhelical density (sigma) of a plasmid, psvo alpha 1p3d, after its introduction into COS-7 cells by DNA tran...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1984
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1984.tb01047.x